›› 2011, Vol. 23 ›› Issue (6): 465-467.doi: 10.3969/j.issn.1004-616x.2011.06.015

• 核辐射安全专家论坛 • Previous Articles     Next Articles

Assessment of radiotherapy damage using hprt gene mutation in cervical cancer patients

WU Li-rui，WANG Lan-peng ，LI Hong-xia，WANG De-hua，SUN Sha   

  1. 1. Department of Gynecology and Obstetrics, China National Petroleum Company Central Hospital, Langfang 065000, Hebei; 2. Department of the Thoracic Surgery, The People’s Hospital Lang Fang, Langfang 065000, Hebei; 3. Department of Gynecology and Obstetrics, General Hospital of Tianjin Medical University, Tianjin 300070, China
  • Received:2011-06-18 Revised:2011-09-27 Online:2011-11-30 Published:2011-11-30
  • Contact: WU Li-rui

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Abstract: OBJECTIVE: To assess the radiotherapy damage using hprt gene mutation in cervical cancer patients. METHOD：Blood samples were collected from 15 cervical cancer patients exposed to radiation at the accumulated dose of 0-60 Gy. hprt gene mutation was studied by multinucleated cell assay. RESULT：Mutation frequency of hprt gene increased after radiation. The frequency increased significantly at dose 30，40 and 50 Gy compared to 0 Gy (P＜0.05 ). At the accumulated dose of 60 Gy，the mutation frequency was also higher than 0 Gy，but not statistically significant. The increase of hprt gene mutation frequency was linear from 0-40 Gy. There was a good dose-response effect between mutation frequency of hprt gene and accumulated dose ( r＝0.900，P＜0.05 ). Analysis showed a linear dose-response with an equation of y＝0.459＋0.014 x ( r＝0.900， P＜0.05). CONCLUSION：Mutation frequency of hprt gene increased after radiation from 0-40 Gy. There was a good dose-response relationship between mutation frequency and accumulated dose. Mutation frequency of hprt gene could be a valuable biodosimeter.

Key words: cervical cancer, hprt gene mutation, radiation-induced damage, dose-response relationship